sofkulturalarniizolyatsiyalashusullari

pervaya polovina xx veka sof kulturalarni izolyatsiyalash usullari bakteriya lb broth (miller) nacl 10 g/l tryptone 10 g/l yeast extract 5 g/l agar agar 15g/l ph 6.8 - 7.2 mpa- meat-peptone agar peptic digest of animal tissue: 5 g/l meat extract: 1.5 g/l yeast extract: 1.5 g/l sodium chloride: 5 g/l agar-agar: 15 g/l ph 7,4 ± 0,2 pepton a k2hpo4 saxaroza mgso4 nacl pepton ph 0,25g/l 5g/l 0,25g/l 0,25g/l 5g/l 7 mikraskopda ko’rish uchun gram usulida bo`yaladi 1. asosiy bo'yoqlardan biri sobit smear ustiga 2-3 daqiqa davomida quyiladi. 2. bo'yoqni to'kib tashlang. smear 1-2 daqiqa davomida lyugol eritmasi bilan yoki yodli eritma bilan gram bo'yicha 1-2 daqiqa davomida preparat qorayguncha quyiladi. 3. eritma tashlanadi, smear rangsizlanmaguncha va oqayotgan suyuqlik …

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