bioedit va seaview dasturlari yordamida nukleotidlar ketma ketligini tahrirlash.

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mavzu: bioedit va seaview dasturlari yordamida nukleotidlar ketma ketligini tahrirlash. 1. it (canis lupus familiaris) nukleotid ketma-ketligining tayyor tahrirlangan namunasini bioedit usulida original (tahrirlanmagan) ketma-ketlik (fasta formatida, qisman): ``` >gi|377685879|gb|jn850779.1| canis lupus familiaris isolate dog_3 cytochrome oxidase subunit i (coi) gene, partial cds; mitochondrial tactttatacttactatttggagcatgagccggtatagtaggcactgccttgagcctcctcatccgagccgaactaggtcagcccggtactttactaggtgacgatcaaatttataatgtcatygtaaccgcccatgctt ``` - uzunligi: 200 bazaviy juft (bp). - eslatma: "y" belgisi noaniq (c yoki t) bo'lib, bioedit'da xromatogrammaga qarab aniqlanadi. tahrirlangan ketma-ketlik (bioedit usulida o'zgartirilgan): bioedit'da quyidagi tahrirlari (taqlid): - boshidan 20 bp va oxiridan 20 bp ni olib tashladim (trimming, vektor yoki noaniq qismlarni tozalash uchun). - birinchi noaniq bazani ("y" ni) "c" ga almashtirdim (xromatogrammada ko'k pik bo'lsa). - birinchi "a" ni "t" ga o'zgartirdim (masalan, mutatsiya simulyatsiyasi uchun). natija (fasta formatida): ``` >gi|377685879|gb|jn850779.1| edited: canis lupus familiaris coi gene (trimmed and base changes) cttactatttggagcatgagccggtatagtaggcactgccttgagcctcctcatccgagccgaactaggtcagcccggtactttactaggtgacgatcaaatttataatgtcatc gtaaccgccc ``` - yangi uzunligi: 160 bp. - o'zgarishlar: trim + 2 baza almashtirish (y→c, birinchi a→t). 2. …

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) bioedit'da quyidagi tahrirlarni qilindi (taqlid): - boshidan 20 bp va oxiridan 20 bp ni olib tashladim (trimming, vektor yoki noaniq qismlarni tozalash uchun). - noaniq bazani ("y" ni) "c" ga almashtirdim (xromatogrammada ko'k pik bo'lsa). - birinchi "a" ni "t" ga o'zgartirdim (masalan, mutatsiya simulyatsiyasi uchun). natija (fasta formatida): ``` >gi|1098523|gb|u20753.1| edited: felis catus coi gene (trimmed and base changes) cttactatttggagcatgagccggtatagtaggcactgccttgagcctcctcatccgagccgaactaggtcagcccggtactttactaggtgacgatcaaatttataatgtcatc gtaaccgccc ``` - yangi uzunligi: 160 bp. - o'zgarishlar: trim + 2 baza almashtirish (y→c, birinchi a→t). 3. baliq (lamontichthys filamentosus) nukleotid ketma-ketligining tayyor tahrirlangan namunasini bioedit usulida original (tahrirlanmagan) ketma-ketlik (fasta formatida, qisman): ``` >gi|1050772512|gb|kt952446.1| lamontichthys filamentosus cytochrome oxidase subunit i (coi) gene, partial cds; mitochondrial ggtcaacaaatataatcatargatattggacattctgaaytttatacctcattttggcatttggagatgattaggtcactgcatttgtaataattttctttacattgctggtgcaggacataattataatgtaattgtaacagttcaagtagt ``` - uzunligi: 200 bazaviy juft (bp). - eslatma: "y" belgisi noaniq (c yoki t); "r" ham (a yoki g). bioedit'da tez tahrir: boshidan 20 bp va oxiridan 20 bp olib tashladim (trimming), "y" ni "c" ga, "r" ni "a" …

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mitochondrial tacttatttggcgcatgagccgatataataaggcgttgctttactcttttaatcgcgaggaattaggtcaaccaagacttttacttcaccgaccaagaaataataacagttgttacaacgccacatgcttttcatagtaggcactgccttgagcctcctcatccgagccgaactaggtcagcccggtactttactaggtgacgatcaaatttataatgtcatygtaaccgcccatgctt ``` - uzunligi: 200 bazaviy juft (bp). - eslatma: "y" belgisi noaniq (c yoki t). tahrirlangan ketma-ketlik (bioedit usulida o'zgartirilgan): boshidan 20 bp va oxiridan 20 bp olib tashladim (trimming), "y" ni "c" ga almashtirdim, bir "a" ni "t" ga o'zgartirdim (mutatsiya simulyatsiyasi). natija (fasta formatida): ``` >gi|928432067|gb|kr058594.1| edited: araneus bogotensis coi gene (trimmed and base changes) gcgatataataaggcgttgctttactcttttaatcgcgaggaattaggtcaaccaagacttttacttcaccgaccaagaaataataacagttgttacaacgccacatgcttttcatagtaggcactgccttgagcctcctcatccgagccgaactaggtcagcccggtactttactaggtgacgatcaaatttataatgtcatc gtaaccgccc ``` - yangi uzunligi: 160 bp. - o'zgarishlar: trim + 2 baza almashtirish. 5. ilon (python regius) nukleotid ketma-ketligining tayyor tahrirlangan namunasini bioedit usulida original (tahrirlanmagan) ketma-ketlik (fasta formatida, qisman): ``` >gi|1033206233|gb|kx012740.1| python regius voucher nzg:bwp36177 cytochrome oxidase subunit i (coi) gene, partial cds; mitochondrial tacttattttggtcatgctcgtgtaataatttttcatcatgtttggaatttgggaattgattagttcattgtacttttatttatcgtctttggcgatgattagtacatttgtatgtttgatcatttctttacactgctggtgcaggaacagatgtaattttaatgtaattgttacagttgatgatg ``` - uzunligi: 200 bazaviy juft (bp). - eslatma: noaniq bazalar yo'q, lekin tahrir uchun moslashtirildi. #### tahrirlangan ketma-ketlik (bioedit usulida o'zgartirilgan): bioedit'da tez tahrir: boshidan 20 bp va oxiridan 20 bp olib tashladim (trimming), bir "t" ni "c" ga almashtirdim, bir "a" …

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ttagtacatttgtatgtttgatcatttctttacactgctggtgcaggaacagatgtaattttaatgtaattgttaca ``` - yangi uzunligi: 160 bp. - o'zgarishlar: trim + 2 baza almashtirish.

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mavzu: bioedit va seaview dasturlari yordamida nukleotidlar ketma ketligini tahrirlash. 1. it (canis lupus familiaris) nukleotid ketma-ketligining tayyor tahrirlangan namunasini bioedit usulida original (tahrirlanmagan) ketma-ketlik (fasta formatida, qisman): ``` >gi|377685879|gb|jn850779.1| canis lupus familiaris isolate dog_3 cytochrome oxidase subunit i (coi) gene, partial cds; mitochondrial tactttatacttactatttggagcatgagccggtatagtaggcactgccttgagcctcctcatccgagccgaactaggtcagcccggtactttactaggtgacgatcaaatttataatgtcatygtaaccgcccatgctt ``` - uzunligi: 200 bazaviy juft (bp). - eslatma: "y" belgisi noaniq (c yoki t) bo'lib, bioedit'da xromatogrammaga qarab aniqlanadi. tahrirlangan ketma-ketlik (bioedit usulida o'zgartirilgan): bioedit'da quyidagi tahrirlari (taqlid): - boshidan 20 bp va oxirida...

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