uzbekistan one health brucellosis workshop

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aphis proposed rule to align bse import regulations to oie uzbekistan one health brucellosis workshop p. ryan clarke, dvm, mph district epidemiologist u.s. department of agriculture animal and plant health inspection service veterinary services april 3-5, 2017 1 diagnosis of animal brucellosis safeguarding animal health 1 diagnosis of brucellosis animal history herd history microbiological culture serologic tests case monitor (epidemiologist) safeguarding animal health same for goats , cattle, sheep, swine 2 diagnosis of brucellosis serologic tests: which tests were conducted? which tests gave positive results? what is the titer as disclosed by the tests? what classes and subclasses of antibodies are being detected by the tests? safeguarding animal health diagnosis of brucellosis differential interpretation of serology: field strain infection strain 19 infection (vaccine) cross-reacting antibodies to other organisms yersinia /pasteurella salmonella leptospira recent vaccination-strain 19 human error safeguarding animal health strain 19 persists and grows….persistent ab titers 4 diagnosis of …

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accination status of herd and titered animals pregnancy status of herd and titered animals ages of positive animals are there any clinical signs present? history of herd and community safeguarding animal health diagnosis of brucellosis microbiological culture safeguarding animal health diagnosis of brucellosis microbiological culture the only definitive proof that brucellosis exists in a herd is the isolation of brucella field strains from the affected animals. brucella abortus biovars 1,2 and 4 brucella abortus strain 19 and strain rb51 brucella suis vs brucella melitensis vs. brucella abortus proper sampling, handling and culture techniques are essential safeguarding animal health diagnosis of brucellosis microbiological culture brucella species can be difficult to grow proper sampling, handling and culture techniques are essential correct tissues tissue handling tissue preservation tissue transportation experienced laboratory safeguarding animal health diagnosis of brucellosis microbiological culture brucella prefers to resides in a certain set of tissues lymph nodes and reproductive …

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ssays can be impacted by: vaccination environmental conditions of test site safeguarding animal health often same antigen solution can be used for serum or milk 17 purpose of serologic testing surveillance for antibodies (ab) as a indicator of infection serologic assays screening tests use highly sensitive assays minimize false negatives low cost—testing a lot of animals confirmatory tests test positive samples with more specific assays minimize false positives higher cost, usually take more time to perform safeguarding animal health national standardized testing protocol 19 classification of brucellosis tests in us testing protocol: screening test buffered acidified plate antigen (bapa) rapid automated presumptive (rap) primary confirmatory test fluorescence polarization assay (fpa) secondary confirmation test complement fixation (cf) supplemental test 8% card elisa plate/standard plate test rivanol tube/standard tube test western blot data from scs not available – 16 = 0 18 = ic data ~ 2.0 m slaughter ~ 96,997 market …

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sensitive tests don’t miss positives. we want a screening test with a high sensitivity 25 specificity example 110 cattle 10 are infected 100 bru free 3/15/2017 26 safeguarding animal health test a traceback herd specificity if 100 are truly non-infected test specificity = 95% 5 test positive (“false” positive) 95 test negative 95 correctly classified by test 5 misclassified by test 3/15/2017 27 safeguarding animal health diagnostic tests diagnostic tests are not perfect indicators of disease tests have a rate of false responses: false negatives = diseased animals that test (-) false positives = healthy animals that test (+) *so test result does not always equal disease status! 3/15/2017 28 safeguarding animal health diagnostic tests tests with high sensitivity: generally used for screening ideal screening tests: high sensitivity, quick low cost high sensitivity---don’t miss positives tests with high specificity: generally used for confirmation high specificity---don’t miss negatives 3/15/2017 29 safeguarding …

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g – improves specificity (reduces false positives) parallel testing – improves sensitivity (reduces false negatives) 3/15/2017 32 series testing (retesting test positives) all test results must be positive in order to be classified as a positive increases specificity decreases sensitivity looking for non-cases (fps) 3/15/2017 33 philosophy = i want to be more sure before classifying a positive. i don’t trust a positive test result. this protocol makes it easier to classify as negative but much more difficult to classify as positive. you keep testing because you don’t believe your positive test results. you are in essence saying, i think the previous test was a false positive so i will test again. this is usually done in very low prevalence or high consequence diagnoses that might result in serious consequences of life or property. 33 series testing (retesting positives) summary: each test in series must be positive for animal to …

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"uzbekistan one health brucellosis workshop" haqida

aphis proposed rule to align bse import regulations to oie uzbekistan one health brucellosis workshop p. ryan clarke, dvm, mph district epidemiologist u.s. department of agriculture animal and plant health inspection service veterinary services april 3-5, 2017 1 diagnosis of animal brucellosis safeguarding animal health 1 diagnosis of brucellosis animal history herd history microbiological culture serologic tests case monitor (epidemiologist) safeguarding animal health same for goats , cattle, sheep, swine 2 diagnosis of brucellosis serologic tests: which tests were conducted? which tests gave positive results? what is the titer as disclosed by the tests? what classes and subclasses of antibodies are being detected by the tests? safeguarding animal health diagnosis of brucellosis different...

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