sampling, storing, and transporting biological material for testing

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powerpoint presentation sampling, storing, and transporting biological material for testing m.b. sharapov, doctor of medical sciences cdc tashkent office 2017 the significance of correct sampling, labeling, storage, and transportation of biological materials for laboratory testing failure to observe the fundamental principles of organizing and conducting the preanalytical phase leads to errors that in 46–55% of cases are the primary cause of erroneous diagnostic results errors caused by unsatisfactory coordination and harmonization of the actions between clinical-epidemiological services and the diagnostic laboratory: - misnamed patient - misidentified sample - erroneously completed forms errors caused by unsatisfactory conditions for the receipt, storage, and transportation of biological materials: hemolyzed serum lipemic serum contamination of samples change in the specific activity of the sample procedure for blood collection using the bd vacutainer® system apply the tourniquet. grasp the needle and, holding it with both hands by the white and colored caps, twist off and …

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e (hemoculture) bottles bottles for taking a hemoculture industrially (commercially) produced bottles can be categorized into two groups: - manual blood culture bottles; - blood culture bottles for automated blood culture systems. the latter differs from the former by the presence of a colorimetric (bact/alert) or fluorescent (bactec 9000) sensor built into the bottom and the presence in the liquid medium of various adsorbent additives to remove potentially toxic organic components, including antibiotics modern automatic hemoculture testing methods make it possible to capture the growth of microorganisms during the first 24 hours of incubation (sensor), which makes it possible within another 24–48 hours to obtain precise identification of the pathogen and its antibiogram bottles for taking a hemoculture bactectm lytic anaerobic bottles contain soybean-casein broth and co2. designed to isolate and culture anaerobic pathogens from blood samples. contain saponin to release phagocytized microorganisms from leukocytes, which increases the isolation rate. …

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ent and reducing its cost and time spent in hospital due to rapid, precise diagnosis bd bactec™ lytic/10 anaerobic lysing agents facilitate diagnosis of microorganisms partially phagocytized by white blood cells support minimal time for identifying facultative anaerobes. compared to a standard anaerobic medium, they significantly increase the identification of microorganisms and reduce the identification time. bactec™ myco/f lytic allows for the identification of yeasts, fungi, and tuberculosis mycobacteria more quickly than in solid media, at a lower cost, lower labor expense, and less time lysing agents facilitate diagnosis of microorganisms partially phagocytized by white blood cells bd bactec™ 9050 diagnosis of sepsis in minimal time—identification of 90% of positive results over the course of the first day. the maximum test protocol takes 5 days. simultaneous testing of 50 samples, and the capability of loading 5–10 new samples daily. maximum number of tests per year: 3600. measurement method: fluorescence. the …

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course of antibiotics, and in a fasting state, blood is collected from the ulnar vein using a butterfly into a special bottle containing growth media for aerobic and anaerobic microorganisms. don the lab coat, mask, and eye protection. don 2 pairs of gloves over the sleeves of the lab coat to prevent skin contact with infectious material. the edge of the gloves must not be located beneath the lab coat sleeve. gloves must be changed: from patient to patient if blood gets on the surface of the gloves seat the patient. prepare all necessary instruments. apply the tourniquet above the elbow. find the vein. prepare the venipuncture site with an alcohol-soaked gauze pad. place the used pad in the polyethylene bag for disposing of biohazardous waste. insert the butterfly or needle into the holder. smoothly insert the vacutainer (vacuum container) tube into the holder, but do not push it as …

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before being sent to the laboratory, blood bottles shall be stored at room temperature for no more than 6 hours or in a refrigerator (2–8°c) for no more than 24 hours. blood collection for rna and dna testing selection and preparation of material for testing blood serum or plasma may be used to isolate rna. serum preparation venous blood collected without anticoagulants into a glass centrifuge tube remains in it at room temperature (15–20°c) for 30 minutes until clot formation is complete. the serum is decanted into a new plastic tube (1.5 ml in volume), holding back the clot with a glass rod, if necessary. plasma preparation venous blood collected with an anticoagulant is immediately stirred by turning the capped tube containing blood at least 5 times. stir without shaking or creating bubbles. the time between application of the tourniquet and the stirring of the blood and anticoagulant must not …

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powerpoint presentation sampling, storing, and transporting biological material for testing m.b. sharapov, doctor of medical sciences cdc tashkent office 2017 the significance of correct sampling, labeling, storage, and transportation of biological materials for laboratory testing failure to observe the fundamental principles of organizing and conducting the preanalytical phase leads to errors that in 46–55% of cases are the primary cause of erroneous diagnostic results errors caused by unsatisfactory coordination and harmonization of the actions between clinical-epidemiological services and the diagnostic laboratory: - misnamed patient - misidentified sample - erroneously completed forms errors caused by unsatisfactory conditions for the receipt, storage, and transportation of biological ...

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